Tests, Bottles, and Preservatives

Test

Analysis 

Matrix 

Method 

Sample Size/ Container 

Preservative*

Holding Time (Days)
Extract./Analysis 

INORGANICS

 

 

 

 

 

 

Alkalinity 

W/WW 

2320B

250 mL/P 

Cool, 4°C 

 

14

Ammonia

W/WW 

350.1

250 mL/P 

Cool, 4°C, H2SO4, pH < 2 

 

28

Biochemical Oxygen Demand (BOD)

W/WW

5210B

1 L/P 

Cool, 4°C 

 

2

Chloride

W/WW

S/SW 

4500_Cl-E

250 mL/P 

Cool, 4°C 

 

28

Chemical Oxygen Demand (COD) 

W/WW 

410.4

250 mL/P 

Cool, 4°C, H2SO4, pH < 2 

 

28

Conductivity 

W/WW 

2510B

250 mL/P 

Cool, 4°C 

 

28

Corrosivity 

W/WW

S/SW 

9045C

250 mL/P

4 oz/P 

Cool, 4°C 

 

7

Cyanide 

Available Cyanide

W/WW

S/SW 

335.4
1677

1 L/P

4 oz/P

NaOH, pH > 12

 

14

Fluoride 

W/WW 

300.0

250 mL/P 

Cool, 4°C 

 

28

Hardness

W/WW

2340B

250 mL/P 

Cool, 4°C 

 

180

Hexavalent Chromium 

W/WW

S/SW 

7196A 

250 mL/P 

4 oz/P

Cool, 4°C 

 

1

Ignitability 

Flash Point

W/WW

S/SW 

1010

250 mL/P 

4 oz/G 

None 

 

7

Mercury

W/WW

S/SW 

245.1
7470

250 mL/P/G

4 oz/P/G 

HNO3, pH < 2

 

28

Metals ICP/GFAA/AA Including Al, Sb, As, Ba, Be, B, Cd, Ca, Cr, Co, Cu, Fe, Pb, Li, Mg, Mn, Mo, Ni, K, Se, Ag, Na, Sr, Tl, V, Zn 

W/WW

S/SW 

200 Series
6010B
6020

6010B
6020

500 mL/P

4 oz/P

HNO3, pH < 2

 

180

TCLP Metals
(except Hg)

W/WW

S/SW 

1311

1 L/P 

Cool, 4°C 

180

180

Nitrate 

W/WW 

4500-NO3-F

250 mL/P 

Cool, 4°C 

 

2

Nitrate + Nitrite 

W/WW 

4500-NO3-F

250 mL/P 

Cool, 4°C, H2SO4, pH < 2 

 

28

Nitrite 

W/WW 

353.2 Mod 

125 mL/P 

Cool, 4°C 

 

2

Oil & Grease 

W/WW

S/SW 

1664

1 L/AG

8 oz/AG 

Cool, 4°C, H2SO4, pH < 2

 

28

Ortho-Phosphorus 

W/WW 

4500-P-F

125 mL/P 

Cool, 4°C, Filter Immediately 

 

2

pH 

W/WW

S/SW 

4500H+  B

250 mL/P 

4 oz/P

Cool, 4°C 

 

ASAP 

Phenolics 

W/WW

S/SW 

420.4

1 L/AG

6 oz/G 

Cool, 4°C, H2SO4, pH < 2

 

28

Reactive Cyanide 

W/WW

S/SW 

7.3.3.2 

250 mL/P 

4 oz/P

Cool, 4°C 

 

7

Reactive Sulfide

W/WW 

7.3.4.2 

250 mL/P 

4 oz/P

Cool, 4°C 

 

7

Sulfide 

W/WW

S/SW 

376.1

9030A 

250 mL/P 

4 oz/P

Cool, 4°C, Zn Acetate/NaOH, pH > 9

 

7

Sulfate 

W/WW

S/SW 

4500-SO4- E

250 mL/P 

4 oz/P

Cool, 4°C 

 

28

Total Kjeldahl Nitrogen 

W/WW 

351.2

1 L/P 

Cool, 4°C, H2SO4, pH < 2 

 

28

Total Dissolved Solids 

W/WW 

2540C

500 mL/P 

Cool, 4°C 

 

7

Total Organic Carbon (TOC) 

W/WW

S/SW 

415.1

9060

250 mL/G

4 oz/G 

Cool, 4°C, H2SO4, pH < 2 

 

28

Total Phosphorus 

W/WW 

SM4500-P

125 mL/P 

Cool, 4°C, H2SO4, pH < 2

 

28

Total Recoverable Petroleum Hydrocarbons (TRPH) 

W/WW 

418.1

1L/AG 

Cool, 4°C, H2SO4, pH < 2

 

28

Total Solids Moisture 

W/WW

S/SW 

2540G

500 mL/P

4 oz/G

Cool, 4°C 

 

7

Total Suspended Solids 

W/WW 

2540D

500 mL/P 

Cool, 4°C 

 

7

Total Volatile Solids 

W/WW 

2540E

250 mL/P 

Cool, 4°C 

 

7

Turbidity 

W/WW 

180.1

250 mL/P 

Cool, 4°C 

 

2

ORGANICS

 

 

 

 

 

 

BTEX and VOCs in Water

W/WW 

8260B 

4 x 40 mL/G 

Cool, 4°C, HCl, pH < 2 

 

14

BTEX and VOCs in Soil

S/SW 

8260B 

Soils Kit

Cool, 4°C, Methanol 

 

14

Herbicides 

W/WW

S/SW 

8151A

1 L/AG

6 oz/AG

Cool, 4°C 

7

14

40

40

Organochlorine Pesticides 

W/WW

S/SW 

8081

1 L/AG

6 oz/AG

Cool, 4°C, pH 5-9

7

14

40

40

Organophosphorus Pesticides 

W/WW

S/SW 

8141A 

1 L/AG

6 oz/AG

Cool, 4°C, pH 5-9

7

14

40

40

PCBs 

W/WW 

8082

1 L/AG

6 oz/AG

Cool, 4°C 

7

14

40

40

Polynuclear Aromatics (PNAs) 

W/WW

S/SW 

8270

1 L/AG

6 oz/AG

Cool, 4°C 

7

14

40

40

Semivolatile Organics 

W/WW

S/SW 

8270 

1 L/AG

6 oz/AG

Cool, 4°C 

7

14

40

40

TCLP Semivolatiles, Pesticides, and Herbicides

W/WW

S/SW

1311

6 oz/G 

Cool, 4°C 

7

14 (leach)
7 (extract)

40

 40

TCLP Volatiles 

W/WW

S/SW 

1311

6 oz/G 

Cool, 4°C 

14 (leach)
7 (extract)

14

 14

*Chemical Preservative on W/WW matrix only.

Abbreviations used in the previous table follow:

Matrix

Container

Preservatives

W

Water

P

Plastic (HDPE)

HCl

Hydrochloric Acid

WW

Wastewater

AG

Amber Glass

HNO3

Nitric Acid

S

Soil/Sediment

G

Glass

NaOH

Sodium Hydroxide

SW

Solid Waste

   

H2SO4

Sulfuric Acid

NOTES:

  1. Sample preservation should be performed during sample collection.
  2. Soil samples can be collected in either glass jars (for organics) or plastic jars (for metals and inorganics).
  3. Extraction holding times are from the date of sampling, and analysis holding times are from the date of extraction.
  4. If analyzing for dissolved metals, the sample shall be field-filtered through a 0.45-µm filter immediately (within 15 minutes) after sample collection and prior to preservation.
  5. Provide twice the number of containers listed when matrix spike, matrix duplicate, and matrix spike duplicate analyses are requested for the sample. Minimum frequency is one per 20 field samples.
  6. This table includes the requirements of the U.S. Environmental Protection Agency, as published in the Code of Federal Regulations, Volume 49, Number 209, 40 CFR 136 dated October 26, 1984, page 43260.